Enterisol Ileitis FF

Company: Boehringer Ingelheim Animal Health

Lawsonia Intracellularis Vaccine

Avirulent Live Culture

For use in swine only

This product has been shown to be effective for the vaccination of healthy swine 3 weeks of age or older against Lawsonia intracellularis. The duration of immunity has been determined to be at least 7 weeks. For more information regarding efficacy and safety data go to productdata.aphis.usda.gov.

In clinical studies the vaccine affected gross and microscopic intestinal lesions of ileitis and colonization by L. intracellularis following challenge with the virulent microorganism.

Directions And Dosage

Administer 1 dose per pig orally or via the drinking water.

Vaccine Preparation

Plan vaccination timing to allow thawing of the contents of the vaccine vial in a trickling cool water bath until liquid (usually 30-40 minutes). Shake well and use entire contents immediately. Do not thaw vaccine by removing from the freezer the day before use. This will inactivate the vaccine.

Vaccination Via The Drinking Water

Conventional Water Directions (open trough or barrel type [tank] system):

1. Do not thaw vaccine until ready to vaccinate.

2. Do not treat animals with medicated feed containing antimicrobials effective against Lawsonia spp. for a minimum of 3 days before and 3 days after the day of vaccination. This will result in a vaccination window totalling 7 days in length.

3. Discontinue sanitizers and disinfectants from drinking water prior to vaccination. Flush watering system with nonchlorinated/nontreated clean water to eliminate any antibacterial agents.

4. Thaw vaccine according to directions.

5. Add number of doses of vaccine equal to or more than the number of pigs to vaccinate, to the appropriate amount of clean, nontreated drinking water (see Caution section).

6. Final solution containing vaccine should be consumed within 6 hours after thawing of vaccine.

Directions for Automatic Watering Systems equipped with proportioner:

Several types of medicators/proportioners are commercially available.

1. Do not thaw vaccine until ready to vaccinate.

2. Do not treat animals with medicated feed containing antimicrobials effective against Lawsonia spp. for a minimum of 3 days before and 3 days after the day of vaccination. This will result in a vaccination window totalling 7 days in length.

3. Provide sufficient watering space so that all pigs can drink within a 6 hour time frame.

4. Discontinue sanitizers and disinfectants from drinking water prior to vaccination. Flush watering system with nontreated, clean water to eliminate any antibacterial agents. (This can be accomplished during pre-measurement of stock solution volume requirement in step 6.)

5. Set proportioner to deliver 1 oz. (30 mL) of vaccine solution per 1 gallon (3.8 liters) of drinking water. Regularly clean proportioners internally to remove antibiotics or other contaminants that may impair vaccine viability. Maintain proportioners to manufacturer’s specifications in order to safeguard accurate vaccine administration.

6. Pre-measure the volume of stock solution required for vaccination during a 6 hour period as follows:

a. Fill a clean bucket or other suitable fluid container with water. Be sure to start with more water than the group of swine is expected to consume in a 6 hour period.

b. Insert proportioner draw tube into the water and ensure that the inlet end rests on the bottom of the container.

c. Adjust valves to start proportioned water flowing into the group of swine scheduled for vaccination.

d. After 6 hours, record the volume of water drawn from the container during this period. This 6 hour disappearance volume will be the total volume of clean water, chlorine neutralizer (step 7c), and vaccine to be used on the day of vaccination.

e. Return water supply flow to normal (bypass the proportioner) until ready to vaccinate.

f. NOTE: It is recommended to conduct this pre-measurement at the same time of day as intended for vaccination.

7. Prepare vaccine stock solution as follows:

a. Thaw vaccine according to directions.

b. Add approximately half of the pre-measured amount of clean, disinfectant-free water to the container to be used for stock solution.

c. To ensure vaccine viability, use one or more Reload Pack™ (Reload Pack™ DT or Reload Pack™ DT 5X), which contain the stabilizer sodium thiosulfate, with any source of drinking and stock solution water. A blue dye is also included to facilitate charging water lines and detection of vaccine-containing water (step 8).

d. Add number of vaccine doses to the stock solution container equal to or greater than the number of pigs to vaccinate. Mix thoroughly.

e. Add clean, disinfectant-free water to bring the total volume of this stock solution to the pre-measured 6 hour volume. Mix thoroughly.

8. Vaccinate pigs using the prepared stock-solution as follows:

a. Insert proportioner draw tube into the vaccine stock solution. Ensure that the inlet end is resting on the bottom of the solution container.

b. Adjust water supply valves to direct water through the proportioner.

c. Charge the main water distribution lines throughout the facility being vaccinated. To do this, drain (bleed) water from the valve or drinker on each main line at the end farthest away from the proportioner.

d. Drain water from each main line until the water appears light blue in color.

e. Pigs should consume the vaccine-containing water within 6 hours after thawing the vaccine. Do not medicate or use disinfectants immediately following vaccination.

Enterisol Ileitis FF Caution

The actual amount of water consumed may vary considerably depending on several factors, including environmental temperature and drinker type. It is recommended to pre-measure the actual stock solution volume requirements the day prior to vaccination, during the planned vaccination time period. This should be done in order to ensure that the vaccine is consumed within the 6 hour recommended time frame.

Precautions

Store frozen at ≤ -60°C. Do not thaw until immediately prior to use. After thawing, shake well and use entire contents immediately. Do not store thawed vaccine. Do not vaccinate within 21 days before slaughter. Do not mix with other products, except as specified on the label. In case of anaphylactoid reaction, administer epinephrine. Do not use in pregnant swine or breeding age boars. Inactivate unused contents before disposal. In case of human exposure, contact a physician.

Incompatibility

Do not vaccinate animals which are receiving treatment with antimicrobials effective against Lawsonia spp. Such antimicrobials should be withheld for a minimum of 3 days before and 3 days after the day of vaccination.

ENTERISOL^® is a registered trademark of Boehringer Ingelheim Vetmedica GmbH, used under license.

RELOAD PACK™ is a trademark of Boehringer Ingelheim Animal Health USA Inc., used under license.

Manufactured by

Boehringer Ingelheim Animal Health USA Inc., St. Joseph, MO 64506

Tel: 1 (888) 637-4251

VLN/PCN 124/10L1.00

Distributed in Canada by

Boehringer Ingelheim Animal Health Canada Inc, Burlington ON L7L 5H4

CPN: 1028118.8